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电针对AD大鼠行为学及海马NOS、CaMKⅡ、BDNF表达的影响

作　者: 张晓东
导　师: 姜国华
学　校: 黑龙江中医药大学
专　业: 人体解剖与组织胚胎学
关键词: Alzheimer’s disease Electro-puncture Memory and learning CaMKⅡ NOS BDNF
分类号: R245
类　型: 硕士论文
年　份: 2011年
下　载: 44次
引　用: 1次
阅　读: 论文下载

内容摘要

Objective:AD is a form of brain damage to memory and cognitive function-based central nervous system degenerative disease symptom of behavioral and psychological abnormaties. Current ly not yet fully understand the causes of AD, Most of the drugs are in the treatment of clinical trials and the long-term use of such medicine easy to cause serious side effect.Therefore from the point of Chinese medical practitioners, to explore the effective treaatment has become the main trends in research. In recent years, clinical reports and experimental studies show that electro-puncture as an important component of Chinese medicine for the prevention and treatment of AD has a positive effect. Therefore, the application intracerebral injection of STZ,the establishment of rat model of AD, study of electro-puncture on learing and memory abilitities AD model rats. Effect of the study on learning and memory ability, hippocampus CaMKⅡ, NOS, BDNF expression,electro-puncture treatment of AD for the clinical application and to provide experimental basis for further research.Methods:Selective healthy Wistar male rats were 60. They were used for Morris test. We select 50 of them. Were randomly divided into normal group,moodel group, model control group,western medicine group and electro-puncture group. Methods of modeling micro-syringe on each side of intracerebraloventricular injection of 10% STZ 10 ul established AD model. Model control group were injected with normal saline instead of STZ.After modeling, electropuncture group after after electropuncture stimulation " Baihui", "Dazhui", "Shenshu", "Zusanli", "Taixi" points for treatment.With the mooris water maze test of learning and memory in rats, one randomly selected 50 ratrs were observed by immunohistoochemistry in hippocampus of rats CaMKⅡ、NO、BDNF expression.Results:(1) Behavioral:Compared with model group, the behavioral of the electropuncture group and weatern medicine group are significantly increased. Escape latency and swimming distance was significantly shorter (P<0.01).Rats in the former platform quadrant swim time, the percentage of the total swimming time was significantly higher. For the first time through the original platfprm in the model group, expression of CaMKⅡ、NO、BDNF was significant obvious in the model group. Compared with the model group, the expressions of electropuncture group and western medicine group were significantly increased. After the treatment, comparsion of the three group were significant difference (P<0.01).Conclusion:(1)Electro-puncture treatment AD rats significantly improved the memory and learning abilities. (2) Electro-puncture treatment can improve CaMKⅡ、NO、BDNF expression in the AD rat brain

全文目录

ABSTRACT  4-6
前言  6-7
文献综述  7-21
  一.AD的流行病学  7
  二.现代医学对AD的认识和研究  7-15
    (一) AD的历史回顾  7-8
    (二) AD的病理学研究  8
    (三) AD病因及发病机制的研究  8-12
    (四) 海马与学习记忆的研究  12-14
    (五) AD的治疗  14-15
  三.AD模型制备的研究进展  15-16
    1. STZ动物模型  15
    2. 以Aβ为基础的动物模型  15-16
    3、Tau蛋白过度磷酸化模型  16
    4、其他  16
  四.中医对AD的认识和治疗研究进展  16-21
    (一) 中医对AD病名的溯源及记载  16-17
    (二) 中医对AD病因病机的认识  17-18
    (三) 中医药治疗AD的研究进展  18-21
材料和方法  21-25
  1.1 实验动物及分组  21
  1.2 实验试剂和仪器  21-22
  1.3 AD模型制备及给药  22
  1.4 大鼠行为学检测  22
  1.5 针刺方法  22-23
  1.6 灌注取材及染色  23-24
  1.7 免疫组织化学法(SP法)  24
  1.8 图像分析及统计学处理  24-25
结果  25-32
  2.1 大鼠行为学态观察  25
  2.2 电针对大鼠学习记忆能力的影响  25-27
  2.3 海马区HE染色的结果  27-28
  2.4 海马区NOS免疫组化染色结果  28
  2.5 海马区CaMKⅡ-α免疫组化染色结果  28
  2.6 海马区BDNF免疫组化染色结果  28-32
讨论  32-41
  一.选穴依据  32-33
  二.电针对AD大鼠行为学治疗意义  33-34
  三 NOS、CaMKⅡ及BDNF与NMDA受体的关系  34-37
  四. NMDA受体与学习记忆关系  37-38
  五.电针对AD大鼠学习记忆改善  38-41
结论  41-42
致谢  42-43
参考文献  43-49
附录  49-53
攻读博、硕士学位期间发表的论文  53-55
个人简历  55

电针对AD大鼠行为学及海马NOS、CaMKⅡ、BDNF表达的影响

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全文目录

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