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牛血清蛋白和鱼精蛋白从NOCC/海藻酸盐水凝胶中的释放：两种不同等电点的生物分子的对照试验

作　者: GOUFO LABOU（妮娜）
导　师: 王征
学　校: 天津大学
专　业: 药剂学
关键词: 等电点羧甲基壳聚糖聚乙二醇聚乙烯吡咯烷酮鱼精蛋白牛血清蛋白 pH-敏感性水凝胶
分类号: R943
类　型: 硕士论文
年　份: 2011年
下　载: 21次
引　用: 0次
阅　读: 论文下载

内容摘要

pH-敏感水凝胶钙交联海藻酸盐/羧甲基壳聚糖的合成中加入水溶性聚合物PEG(聚乙二醇)和/或PVP(聚乙烯吡咯烷酮),所制备的凝胶通过物理包埋的方法分别载入两种生物分子蛋白:牛血清蛋白和鱼精蛋白。由于两种药物有不同的等电点(分别为4.5和10),溶胀试验和释药试验对于试验介质的pH值的变化、干燥过程、水凝胶中PEG、PVP的含量进行相应调整。凝胶组成中海藻酸盐和羧甲基壳聚糖的重量比为3:3,随着释放介质的pH值由1.2逐渐增长到7.4,两种生物分子的释放率都增长了。pH值的增长对鱼精蛋白影响更为显著,这是由于它的等电点10.0比牛血清蛋白的等电点4.5要高很多。自然干燥的凝胶粒子和冷冻干燥的相比,溶胀率和释药速率都降低。PVP的加入对于凝胶粒子在酸性介质(pH1.2)中的减少突释作用更明显,此外,当加入体积分数为1%的PVP,水凝胶对药物的吸附稍有增加。随着PVP含量的增加,水凝胶对鱼精蛋白和牛血清蛋白的吸附减少。PEG的加入对水凝胶的特性没有显著影响。海藻酸盐/羧甲基壳聚糖凝胶和高等电点蛋白质间的相互作用可以进一步加以研究,从而开发新的蛋白质给药载体。

全文目录

摘要  3-4
ABSTRACT  4-8
CHAPTER 1 Introduction and motivation  8-14
  1.1 Hydrogels  8-11
    1.1.1 pH-sensitive hydrogels  10-11
  1.2 Proteins and the concept of Isoelectric point (pI or IEP)  11-14
CHAPTER 2 Literature reviews  14-35
  2.1 Alginate  14-25
    2.1.1 Source and applications  14-15
    2.1.2 Composition, sequence and molecular weight  15-17
    2.1.3 Physical properties  17-21
    2.1.4 Alginate gels  21-23
    2.1.5 Alginate beads  23-25
  2.2 Chitosan  25-33
    2.2.1 Sources and applications  25-28
    2.2.2 Water-soluble derivatives of chitosan at neutral pH  28-33
      2.2.2.1 Quaternized derivatives of chitineous substances  29-30
      2.2.2.2 N-sulfonated derivatives of chitosan  30
      2.2.2.3 Miscellaneous derivatives  30-32
      2.2.2.4 Carboxyalkylated derivatives of chitosan  32-33
  2.3 Present project  33-35
CHAPTER 3 Characterization of the pH-sensitive calcium-Alginate-NOCC hydrogel and the release profiles of the two drugs: BSA and Protamine  35-57
  3.1 Principle  35-36
    3.1.1 The Interpenetrating Polymeric Network  35-36
  3.2 Bovine Serum Albumin and Protamine  36-39
    3.2.1 Protamine  36-38
    3.2.2 Bovine Serum Albumin (BSA)  38-39
  3.3 Experimental  39-44
    3.3.1 Materials  39-40
    3.3.2 Preparation of calcium–alginate–NOCC beads  40-41
    3.3.3 Scanning electron microscopy (SEM)  41
    3.3.4 Bead diameter analysis  41
    3.3.5 Swelling studies  41-42
    3.3.6 Protein encapsulation  42
    3.3.7 Drug content and loading capacity determination  42-43
    3.3.8 Drugs release studies  43-44
    3.3.9 Differential scanning calorimetry (DSC)  44
  3.4 Results and discussion  44-55
    3.4.1 Characterization of beads  44-46
    3.4.2 Swelling studies  46-48
    3.4.3 Drug release behavior  48-53
    3.4.4 Differential Scanning Calorimetry  53-55
  3.5 Conclusion  55-57
CHAPTER 4 Influence of different amounts of PEG and PVP on the swelling properties of calcium-alginate-NOCC hydrogel and release characteristics of Protamine and BSA  57-74
  4.1 Principle  57-59
    4.1.1 Polyethylene glycols (PEG)  57-58
    4.1.2 Polyvinylpyrrolidone (PVP)  58-59
  4.2 Experimental  59-63
    4.2.1 Materials  59-60
    4.2.2 Preparation of calcium–alginate–NOCC-PEG and/or PVP beads  60-61
    4.2.3 Scanning electron microscopy (SEM)  61
    4.2.4 Swelling studies  61
    4.2.5 Protein encapsulation  61-62
    4.2.6 Drug loading capacity determination  62
    4.2.7 Drugs release studies  62-63
  4.3 Results and discussion  63-73
    4.3.1 Scanning electron microscopy (SEM)  63-64
    4.3.2 Swelling studies  64-66
    4.3.3 Loading capacities  66-68
    4.3.4 Release profiles of BSA and Protamine from the hydrogel beads  68-72
    4.3.5 Release of BSA from Air and freeze-dried calcium-alginate-NOCC-PVP beads  72-73
  4.4 Conclusion  73-74
CHAPTER 5 Concluding remarks  74-76
REFERENCES  76-84
ACKNOWLEDGEMENTS  84

牛血清蛋白和鱼精蛋白从NOCC/海藻酸盐水凝胶中的释放：两种不同等电点的生物分子的对照试验

内容摘要

全文目录

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